Clerodendrum trichotomum leaf extract prevents potassium oxonate-induced hyperuricemia in mice

Ji Hye Kim, Hana Song, Mi-Kyeung Jang, Jung Min Oh, Hee Chul Ko, Se Jae Kim

Abstract


Clerodendrum trichotomum is a folk medicine exhibiting anti-hypertension, anti-arthritis, and anti-rheumatism properties. However, little is known about whether the material might prevent hyperuricemia and associated inflammation. The levels of polyphenols, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging activities, and xanthine oxidase (XO) inhibitory activity are higher in C. trichotomum leaf extract (CTE) than in other extracts. Thus, we explored whether CTE prevented hyperuricemia induced by potassium oxonate (PO) in mice. CTE (400 mg/kg body weight) significantly reduced the serum uric acid (UA), blood urea nitrogen (BUN), and serum creatinine levels and increased urine UA and creatinine levels. CTE ameliorated PO-induced inflammation and apoptosis by reducing the levels of relevant proteins in kidney tissues. Kidney transcriptome analysis showed that CTE enhanced the expression of genes countering the inflammatory response and positively regulated the expression of genes involved in immune system function, ion transport, and metabolic processes. Together, the results show that CTE effectively prevents hyperuricemia and associated inflammation.


Keywords


Clerodendrum trichotomum; hyperuricemia, potassium oxonate, transcriptome, uric acid

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