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MiR-152 promotes Schwann Cell Migration by Targeting CAND1

Feng Han, Wei Qu, Yongjun Rui


MicroRNAs (miRNAs) regulates gene expression at the post-transcriptional level in multiple cellular biology processes. However, the roles of miR-152 in Schwann cells (SCs) are not fully understood. The expressionof miR-152 and cullin-associated and neddylation-dissociated 1 (CAND1) in the injured nerve was detected by using qRT-PCR . The expression of miR-152 and CAND1 in SCs was detected by using Western blot and qRT-PCR. Cell migration ability was determined by Transwell assay. The association between microRNA-152 and CAND1 were verified by Luciferase reporter gene and RNA immunoprecipitation (RIP) assay. In the current study, firstly, On the 1st, 4th, 7th and 14th day after nerve injury, the levels of miR-152 mRNA in proximal and distal nerve segments increased significantly. Moreover, overexpression of miR-152 led to the promotion of migration in SCs, while a remarkably repression of migration was observed in the cell which were transfected with miR-152 inhibitor. We also found that CAND1 was a target gene of miR-152. Furthermore, the expressionlevel of CAND1 mRNA in the proximal and distal nerve segment was strikingly reduced after sciatic nerve injury. Importantly, the expressions of CAND1 was negatively regulated by miR-152. Besides, CAND1 upregulation blocked the miR-152 overexpression-mediated promotion effect on migration in SCs. Our data supported miR-152 promoted SC migration by inhibiting CAND1.


MicroRNAs ● Cell Migration Assays ● Schwann cells

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Carr MJ, Johnston AP. (2017) Schwann cells as drivers of tissue repair and regeneration. Curr Opin Neurobiol 47: 52-57.

Gu Y, Chen C, Yi S, Wang S, Gong L, Liu J, Gu X, Zhao Q, Li S

. (2015) miR-sc8 Inhibits Schwann Cell Proliferation and Migration by Targeting Egfr. PLoS One 10: e0145185.

Jessen KR, Mirsky R, Lloyd AC. (2015) Schwann Cells: Development and Role in Nerve Repair. Cold Spring Harb Perspect Biol 7: a020487.

Jessen KR, Mirsky R. (2016) The repair Schwann cell and its function in regenerating nerves. J Physiol 594: 3521-3531.

Jiang L, Jones S, Jia X. (2017) Stem Cell Transplantation for Peripheral Nerve Regeneration: Current Options and Opportunities. Int J Mol Sci 18: pii: E94.

Kang M, Li Y, Zhao Y, He S, Shi J. (2018) miR-33a inhibits cell proliferation and invasion by targeting CAND1 in lung cancer. Clin Transl Oncol 20: 457-466.

Korzeniewski N, Hohenfellner M, Duensing S. (2012) CAND1 promotes PLK4-mediated centriole overduplication and is frequently disrupted in prostate cancer

. Neoplasia 14: 799-806.

Li B, Xie Z, Li B. (2016) miR-152 functions as a tumor suppressor in colorectal cancer by targeting PIK3R3. Tumour Biol 37: 10075-10084.

Li J, Zhang W, Zhou M, Kooger R, Zhang Y. (2013) Small molecules modulating biogenesis or processing of microRNAs with therapeuticpotentials. Curr Med Chem 20: 3604-3612.

Ma J, Yao Y, Wang P, Liu Y, Zhao L, Li Z, Li Z, Xue Y. (2014) MiR-152 functions as a tumor suppressor in glioblastoma stem cells by targeting Krüppel-like factor 4. Cancer Lett 355: 85-95.

Monk KR, Feltri ML, Taveggia C. (2015) New insights on Schwann cell development. Glia 63: 1376-1393.

Murata T, Takayama K, Katayama S, Urano T, Horie-Inoue K, Ikeda K, Takahashi S, Kawazu C, Hasegawa A, Ouchi Y, Homma Y, Hayashizaki Y, Inoue S. (2010) miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression. Prostate Cancer Prostatic Dis 13: 356-361.

Musumeci G, Leggio GM, Marzagalli R, Al-Badri G, Drago F, Castorina A

. (2018) Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System. Int J Mol Sci 19: pii: E981.

Panagopoulos GN, Megaloikonomos PD, Mavrogenis AF. (2017) The Present and Future for Peripheral Nerve Regeneration. Orthopedics 40:e141-e156.

Qian T, Zhao L, Wang J, Li P, Qin J, Liu YS, Yu B, Ding F, Gu XS, Zhou SL

. (2016) miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1. Neural Regen Res 11: 1001-1005.

Sullivan R, Dailey T, Duncan K, Abel N, Borlongan CV. (2016) Peripheral Nerve Injury: Stem Cell Therapy and Peripheral Nerve Transfer. Int J Mol Sci 17: pii: E2101.

Sullivan TB, Robert LC, Teebagy PA, Morgan SE, Beatty EW, Cicuto BJ, Nowd PK, Rieger-Christ KM, Bryan DJ. (2018) Spatiotemporal microRNA profile in peripheral nerve regeneration: miR-138 targets vimentin and inhibits Schwann cell migration and proliferation. Neural Regen Res 13: 1253-1262.

Wang F, Ying H, He B, Pan Y, Sun H, Wang S. (2016) Circulating miR-148/152 family as potential biomarkers in hepatocellular carcinoma. Tumour Biol 37: 4945-4953.

Xie D, Liang Y, Su Y, An Y, Qu P. (2018) miR -152 inhibits proliferation of human endometrial cancer cells via inducing G2/M phase arrest by suppressing CDC25B expression. Biomed Pharmacother 99: 299-305.

Yao C, Shi X, Zhang Z, Zhou S, Qian T, Wang Y, Ding F, Gu X. (2016) Hypoxia-Induced Upregulation of miR-132 Promotes Schwann Cell Migration After Sciatic Nerve Injury by Targeting PRKAG3. Mol Neurobiol 53: 5129-5139.

Yao C, Wang Y, Zhang H, Feng W, Wang Q, Shen D, Qian T, Liu F, Mao S, Gu X, Yu B. (2018) lncRNA TNXA-PS1 Modulates Schwann Cells by Functioning As a Competing Endogenous RNA Following Nerve Injury. J Neurosci 38: 6574-6585.

Yi S, Wang QH, Zhao LL, Qin J, Wang YX, Yu B, Zhou SL

. (2017) miR-30c promotes Schwann cell remyelination following peripheral nerve injury. Neural Regen Res 12: 1708-1715.

Yi S, Wang S, Zhao Q, Yao C, Gu Y, Liu J, Gu X, Li S. (2016) miR-sc3, a Novel MicroRNA, Promotes Schwann Cell Proliferation and Migration by Targeting Astn1. Cell Transplant 25: 973-982.

Yi S, Yuan Y, Chen Q, Wang X, Gong L, Liu J, Gu X, Li S. (2016) Regulation of Schwann cell proliferation and migration by miR-1 targeting brain-derived neurotrophic factor after peripheral nerve injury. Sci Rep 6: 29121.

Yu B, Zhou S, Yi S, Gu X.(2015) The regulatory roles of non-coding RNAs in nerve injury and regeneration. Prog Neurobiol 134: 122-139.

Zhang P, Sun H2, Yang B, Luo W, Liu Z, Wang J, Zuo Y. (2017) miR-152 regulated glioma cell proliferation and apoptosis via Runx2 mediated by DNMT1. Biomed Pharmacother 92: 690-695.

Zhang X, Gong X, Qiu J, Zhang Y, Gong F. (2017) MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells.Exp Ther Med, 2017; 14: 2809-2816.

Zhou S, Gao R, Hu W, Qian T, Wang N, Ding G, Ding F, Yu B, Gu X

. (2014) MiR-9 inhibits Schwann cell migration by targeting Cthrc1 following sciatic nerve injury. J Cell Sci 127: 967-976.

Zhu H, Xue C, Yao M, Wang H, Zhang P, Qian T, Zhou S, Li S, Yu B, Wang Y, Gu X. (2018) miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury. Cell Death Dis 9: 720.


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