MiR-152 promotes Schwann Cell Migration by Targeting CAND1

Feng Han, Wei Qu, Yongjun Rui

Abstract


MicroRNAs (miRNAs) regulates gene expression at the post-transcriptional level in multiple cellular biology processes. However, the roles of miR-152 in Schwann cells (SCs) are not fully understood. The expressionof miR-152 and cullin-associated and neddylation-dissociated 1 (CAND1) in the injured nerve was detected by using qRT-PCR . The expression of miR-152 and CAND1 in SCs was detected by using Western blot and qRT-PCR. Cell migration ability was determined by Transwell assay. The association between microRNA-152 and CAND1 were verified by Luciferase reporter gene and RNA immunoprecipitation (RIP) assay. In the current study, firstly, On the 1st, 4th, 7th and 14th day after nerve injury, the levels of miR-152 mRNA in proximal and distal nerve segments increased significantly. Moreover, overexpression of miR-152 led to the promotion of migration in SCs, while a remarkably repression of migration was observed in the cell which were transfected with miR-152 inhibitor. We also found that CAND1 was a target gene of miR-152. Furthermore, the expressionlevel of CAND1 mRNA in the proximal and distal nerve segment was strikingly reduced after sciatic nerve injury. Importantly, the expressions of CAND1 was negatively regulated by miR-152. Besides, CAND1 upregulation blocked the miR-152 overexpression-mediated promotion effect on migration in SCs. Our data supported miR-152 promoted SC migration by inhibiting CAND1.


Keywords


MicroRNAs ● Cell Migration Assays ● Schwann cells

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