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Breaking the sporoderm of Ganoderma lucidum spores by combining enzymatic hydrolysis reaction with physical actuation

Yan Liu, Peiwen Mao, Mengyuan Jin, Liu-Dingji Li, Xiaohui Bai, Xuanwei Zhou


Ganoderma lucidum spores (GLS), ovoid generative cells, were a traditional health care and nutrition. Breaking the spore wall of Ganoderma is beneficial to the absorption of its nutrients. Nowadays various sporoderm-broken methods have been applied for breaking the sporoderm of GLS. Little is well known, however, relevant biological methods using enzymes to digest the cell wall polymers. In this study, a qualitative plate assay method was employed to screen the G. lucidum strain with a capable of secreting manganese peroxidase (MnP). After induced culture of the Ganoderma mycelia for the production of enzymes, the supernatants designated as crude enzyme solutions were analyzed on its enzyme activity using a spectroscopy method. Furthermore, GLS was treated by the supernatants coupled with high pressure homogenization technique. Effects of sporoderm-broken were evaluated with an optical microscope method, while the contents of polysaccharides and triterpenes in the completely sporoderm-broken spores were examined by HPLC. The results showed that Ganoderma strains could produce a MnP with higher activities, which could be used for the breaking the sporoderm of GLS. Compared with sporoderm-unbroken spores, polysaccharides and triterpenes in the sporoderm-broken spores were obviously increased by using the culture supernatants for breaking the cell walls of GLS. In conclusion, a new strategy for breaking the sporoderm of GLS was developed, which has potential applications to large-scale production of sporoderm-broken GLS.


Ganoderma lucidum Manganese peroxidase(MnP), Spore, Sporoderm-broken. Bioactive component

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Carlsson N, Borde A, Wölfel S, Åkerman B, Larsson A (2011). Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers. Analytical Biochemistry 411: 116-121.

Chen XL, Liu XC, Sheng D, Huang D, Li WZ, Wang X (2012). Distinction of broken cellular wall Ganoderma lucidum spores and G. lucidum spores using FTIR microspectroscopy. Spectrochim Acta Part A: Molecular and Biomolecular Spectroscopy 97:667-672.

Chen Y, Sun D, Zhou Y, Liu L, Han W, Zheng B, Wang Z, Zhang Z (2014). Cloning, expression and characterization of a novel thermophilic polygalacturonase from Caldicellulosiruptor bescii DSM 6725. International Journal of Molecular Sciences 3:5717-5729.

Daly R, Hearn MT (2005). Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. Journal of Molecular Recognition 18: 119-138.

Day BJ, Kariya C (2005). A novel class of Cytochrome P450 Reductase Redox Cyclers: Cationic Manganoporphyrins. Toxicological Sciences 85:713-719.

Delroisse JM, Dannau M, Gilsoul JJ, El Mejdoub T, Destain J, Daniel P, Thonart P, Haubruge E, Vandenbol M (2005). Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris. Protein Expression and Purification 42: 286-294.

Eibes G, Lú-Chau T, Feijoo MG, Moreira T, Lema JM (2005). Complete degradation of anthracene by Manganese Peroxidase in organic solvent mixtures. Enzyme and Microbial Technology 37: 365-372.

Gao WC, Li QY, Xian RQ, Yu HY, Wang XB (2014). Determination of residual solvents in broken cellular wall of Ganoderma lucidum spore powder by DI-GC. Journal of Pharmaceutical Research 33: 642-644.

Hatvani N, Mécs I (2002). Effect of the nutrient composition on dye decolorisation and extracellular enzyme production by Lentinus edodes on solid medium. Enzyme and Microbial Technology 30: 381-386.

Hong F, Meinander NQ, Jönsson LJ (2002). Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioengineering 79: 438-449.

Huang XL, Wu HQ, Huang F, Lin XS (2006). Analys of polysaccharide from broken cellular wall and unbroken spore of Ganoderma lucidum. Chinese Traditional and Herbal Drug 37: 813-816, abstr.

Jin H, Jin F, Jin JX, Xu J, Tao TT, Liu J, Huang HJ (2013). Protective effects of Ganoderma lucidum spore on cadmium hepatotoxicity in mice. Food and Chemical Toxicology 52: 171-175.

Li J, Liu DB, Xia ZL, Tu HQ (2009). Determination of polysaccharides content in Ganoderma lucidum by spectrometry. Hunan Agricultural Sciences 2: 34-38, abstr.

Li QZ, Chang YZ, Su KQ, Wang XL, Bai XH, Zhou XW (2018). Cloning and characterization of the promoters of fungal immunomodulatory proteins gene from Ganoderma spp. and their response to methyl jasmonate and salicylic acid. International Journal of Medical Mushrooms 20:177-189.

Li SF, Zhang ZJ, Li HD (2008). Study on wall- broken technology of Ganoderma lucidum spores. Science and Technology of Food Industry 9: 195-199, abstr.

Liu JY, Cai YJ, Liao XR, Wang X, Zhang DB (2010). Screening and optimization of laccase production from a laccase producing fungal. Food and Machnery 26: 10-14.

Liu MR, Fu SY, Zhan HY, Lin L (2006). Progress of researches on applications of manganese peroxidase. Chemistry and Industry of Forest Products 26: 112-116, abstr.

Liu X, Yuan JP, Chuang CK, Chen XJ (2002). Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum. Cancer Letters 182:155-161.

Shan WH, Zhang L, Shi YZ, Liang RX, Shang LW, Hu QW (2000). Quantitative determination of ganodermic polysaccharide and total saccharide in Lingzhi fermented liquid. Lishizhen Medicine and materia Medica Research 11: 797-799, abstr.

Shanmugam S, Rajasekaran P, Thanikal JV (2009). Synthetic dye decolourization, textile dye and paper industrial effluent treatment using white rot fungi Lentines edodes. Desalination and Water Treatment 4: 143-147, abstr.

Wu CH, Li Y, Guo XL, Yang JJ, Qiu LM (2011). Discussion on the determination of the total content of triterpene in Ganoderma by spectrophotometry. China Licensed Pharmacist 4: 29-32, abstr.

Xia ZL, Wang CH, Jiang XJ, LI YW, Xong XY (2005). A study on the enzyme sporderm-broken techniques of Ganoderma lucidum spores. Acta Edulis Fungi 12: 14-18, abstr.

Xu H, Guo MY, Jin T, Gao YH, Lu HL, Li W, Zhou XW (2016). Optimization of fermentation conditions of recombinant Pichia pastoris for the production of Ganoderma lucidum manganese peroxidase. Academic Journal of Second Military Medical University 37: 955-962.

Yang K, Liu F, Lian XJ, Zou L, Guo XH, Yu T (2017). Research Progress on Wall-broken Methods of Ganoderma Lucidum Spores and Its Pharmacological Effects. Journal of Chengdu University(Natural Science) 36:364-368, abstr.

Yang XY, Zhu JP (2010). Comparison study on the contents of triterpene between the G. lucidum spore powder with broken and unbroken cellular wall comparison study on the content of triterpenes. Chinese Journal of Pharmaceutical Analysis 30: 2227-2228.

Zhang SQ, Zhu JJ, Wang CZ, Sun YC (2004). Development of wall breaking method of Ganoderma lucidum spores. Transactions of The Chinese Society of Agricultural Machinery 35: 160-162, abstr.

Zhao D, Chang MW, Li JS, Suen W, Huang J (2014). Investigation of ice-assisted sonication on the microstructure and chemical quality of Ganoderma lucidum spores. Journal of Food Science 79: E2253-E2265.

Zhao JS, Wei L, Yu YP, Wang X, Tan RD, Ao L (2013). Research progress on the test method of Reishi mushroom powder broken rate. Guide of China Medicine 11: 431-434, abstr.

Zhou XW (2014). Molecular cloning and heterologous expression in Pichia pastoris of two genes related to lignin modifying enzymes from Ganoderma spp. Shanghai Normal University.

Zhou XW, Su KQ, Zhang YM (2012). Applied modern biotechnology for cultivation of Ganoderma and development of their products. Appllied Microbiology and Biotechnology 93: 941-963.


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