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Breaking the sporoderm of Ganoderma lucidum spores by combining enzymatic hydrolysis reaction with physical actuation

Yan Liu, Peiwen Mao, Mengyuan Jin, Liu-Dingji Li, Xiaohui Bai, Xuanwei Zhou

Abstract


Ganoderma lucidum spores (GLS), ovoid generative cells, were a traditional health care and nutrition. Breaking the spore wall of Ganoderma is beneficial to the absorption of its nutrients. Nowadays various sporoderm-broken methods have been applied for breaking the sporoderm of GLS. Little is well known, however, relevant biological methods using enzymes to digest the cell wall polymers. In this study, a qualitative plate assay method was employed to screen the G. lucidum strain with a capable of secreting manganese peroxidase (MnP). After induced culture of the Ganoderma mycelia for the production of enzymes, the supernatants designated as crude enzyme solutions were analyzed on its enzyme activity using a spectroscopy method. Furthermore, GLS was treated by the supernatants coupled with high pressure homogenization technique. Effects of sporoderm-broken were evaluated with an optical microscope method, while the contents of polysaccharides and triterpenes in the completely sporoderm-broken spores were examined by HPLC. The results showed that Ganoderma strains could produce a MnP with higher activities, which could be used for the breaking the sporoderm of GLS. Compared with sporoderm-unbroken spores, polysaccharides and triterpenes in the sporoderm-broken spores were obviously increased by using the culture supernatants for breaking the cell walls of GLS. In conclusion, a new strategy for breaking the sporoderm of GLS was developed, which has potential applications to large-scale production of sporoderm-broken GLS.


Keywords


Ganoderma lucidum Manganese peroxidase(MnP), Spore, Sporoderm-broken. Bioactive component

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References


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