Gold nanoparticles, nanoinsulin preparation and its dose dependent effect on trace elements in Alloxan induced diabetic mice

Bashir Ahmad, Manzoor Ahmad, Muhammad Nasir Khan Khattak, Mohamed Y. Zaky, Gehad R. Abdelbaset, Zaheer Ud Din, Akbar Husain, Syed Rafiq Hussain, Bakhtawar Khan, Pengyu Su, Mohammed Alshwmi, Ghulam Nabi, Yaser Gamallat, Faizur Rahman, Ata Ur Rehman, Tariq Mehmood

Abstract


Diabetes Mellitus (DM) is an endocrine disorder. Different types of nanoparticles are used as drug and insulin carriers. However, these nanoparticles also induce toxicity. Therefore, in the current study, we prepared GNPs, characterize it through SEM, XRD, and prepare its nanoinsulin (NI), administered dose dependently to the DM. The trace elements like Zn, Co, Pb, Cd and Cu were measured using FAAS. The SEM and XRD results showed that the prepared sample was GNPs with 6 nm size. The level of Cd and Co significantly (p<0.05) decreased in lung tissues of nanoinsulin treated mice (NIT) as compared to normal (NC), diabetic (DC) and insulin treated mice (INT). The cobalt level increased significantly (p<0.05) in liver tissues of NIT compared to all groups. NI did not alter the trace element level in kidney tissues. Taken together, all these information suggest that the NI may be useful for diabetes treatment.


Keywords


Diabetes Mellitus, trace elements, Diabetic mice, nanoinsulin particles, organs weight

References


Chistiakov, D. A., Voronova, N. V. (2009) Zn(2+)-transporter-8: a dual role in diabetes. BioFactors (Oxford, England) 35, 356-363.

Kumar PJ, M., C. (2002) Textbook of Clinical Medicine. Pub: Saunders(London), 1099-1121.

Guariguata, L., Whiting, D. R., Hambleton, I., Beagley, J., Linnenkamp, U., Shaw, J. E. (2014) Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes research and clinical practice 103, 137-149.

Menegazzo, L., Albiero, M., Avogaro, A., Fadini, G. P. (2012) Endothelial progenitor cells in diabetes mellitus. BioFactors (Oxford, England) 38, 194-202.

Kunisaki, M., Bursell, S. E., Umeda, F., Nawata, H., King, G. L. (1998) Prevention of diabetes-induced abnormal retinal blood flow by treatment with d-alpha-tocopherol. BioFactors (Oxford, England) 7, 55-67.

DiSanto, R. M., Subramanian, V., Gu, Z. (2015) Recent advances in nanotechnology for diabetes treatment. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 7, 548-564.

Tiwari, P. M., Vig, K., Dennis, V. A., Singh, S. R. (2011) Functionalized gold nanoparticles and their biomedical applications. Nanomaterials 1, 31-63.

Cai, W., Gao, T., Hong, H., Sun, J. (2008) Applications of gold nanoparticles in cancer nanotechnology. Nanotechnology, science and applications 2008.

Dai, L., Banta, G. T., Selck, H., Forbes, V. E. (2015) Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta. Marine environmental research 111, 99-106.

Wong-Ng, W., McMurdie, H. F., Hubbard, C. R., Mighell, A. D. (2001) JCPDS-ICDD Research Associateship (Cooperative Program with NBS/NIST). Journal of research of the National Institute of Standards and Technology 106, 1013-1028.

Sani Usman, M., Hussein, M. Z., Fakurazi, S., Masarudin, M. J., Ahmad Saad, F. F. (2017) Gadolinium-Doped Gallic Acid-Zinc/Aluminium-Layered Double Hydroxide/Gold Theranostic Nanoparticles for a Bimodal Magnetic Resonance Imaging and Drug Delivery System. Nanomaterials (Basel, Switzerland) 7.

Eisenstein, D. J., Weinberg, D. H., Agol, E., Aihara, H., Prieto, C. A., Anderson, S. F., Arns, J. A., Aubourg, Ã. r., Bailey, S., Balbinot, E. SDSS-III: Massive spectroscopic surveys of the distant universe, the Milky Way, and extra-solar planetary systems. The Astronomical Journal 142, 72.

Bhumkar, S., Mehta, M., Shoemaker, A., (2007) Methods and systems for enhancing internet experiences using previews, Google Patents.

Subramani, K. (2009) 8 NPDDS for the Treatment of Diabetes. Drug Delivery Nanoparticles Formulation and Characterization 191, 117.

Shukla, R., Bansal, V., Chaudhary, M., Basu, A., Bhonde, R. R., Sastry, M. (2005) Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir 21, 10644-10654.

Chithrani, B. D., Chan, W. C. W. (2007) Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Letters 7, 1542-1550.

Pan, Y., Neuss, S., Leifert, A., Fischler, M., Wen, F., Simon, U., Schmid, G., Brandau, W., Jahnenâ€Dechent, W. (2007) Size dependent cytotoxicity of gold nanoparticles. Small 3, 1941-1949.

Giacconi, R., Cai, L., Costarelli, L., Cardelli, M., Malavolta, M., Piacenza, F., Provinciali, M. (2017) Implications of impaired zinc homeostasis in diabetic cardiomyopathy and nephropathy. BioFactors (Oxford, England) 43, 770-784.

Shalabney, A., Abdulhalim, I. (2012) Figure-of-merit enhancement of surface plasmon resonance sensors in the spectral interrogation. Optics letters 37, 1175-1177.

Wang, H., Sen, S., Elgohary, A., Farid, M., Youssef, M., Choudhury, R. R., ACM, pp. 197-210.

Zordoky, B. N. M., Anwar-Mohamed, A., Aboutabl, M. E., El-Kadi, A. O. S. (2011) Acute doxorubicin toxicity differentially alters cytochrome P450 expression and arachidonic acid metabolism in rat kidney and liver. Drug Metabolism and Disposition 39, 1440-1450.


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