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Therapeutic activities of Puerarin against cancer

Bashir Ahmad, Gehad R. Abdelbaset, Mohamed Y. Zaky, Pengyu Su, Ata Ur Rehman, Bakhtawar Khan, Manzoor Ahmad, Zaheer Ud Din, Mohammed Alshwmi, Akbar Husain, Syed Rafiq Hussain


Isoflavone are the isoflavenoids which are naturally found in plants family Fabaceae (bean, or Leguminosae) mostly occur in the form of glycosides and many of them act as a phytoestrogen in the mammal's body. Traditional Chinese medicine Puerarin (Pue) belong to isoflovene glycoside group, derived from Pueraria lobata (Willd.)  Ohwi and Pueraria thomsonii Benth and approved in 1993 by the Chinese Ministry of Health for treatment of different diseases, but later its anti-cancer activity was also reported. Therefore, in this review for the first time we compile all anti-cancer mechanistic studies of Puerarin (Pue) and provides comprehensive information of Pue against different cancers in in vivo and in vitro studies. Poe induces apoptosis in different cancers, including Non-Small Cell lung cancer (NSCLC), leukemic, oesophageal, gastric, colon, colorectal, hepatocellular and breast cancers. Therefore, this review will provide the clues for future research and clinical treatment.


Puerarin, Pueraria lobata , Pueraria thomsonii, NSCLC, leukemic, oesophageal, gastric, colon, colorectal, hepatocellular, breast cancer

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Adams, N. R. (1995). Detection of the effects of phytoestrogens on sheep and cattle. J Anim Sci, 73(5), 1509-1515.

Bai, M., Wu, Z., & Wang, X. (2010). Advances in research on adjuvant effects applying Traditional Chinese Medicine in cancer chemotherapy. J Modern Oncol, 18, 597-601.

Chen, R., Xue, J., & Xie, M. (2012). Puerarin prevents isoprenaline-induced myocardial fibrosis in mice by reduction of myocardial TGF-beta1 expression. J Nutr Biochem, 23(9), 1080-1085. doi:10.1016/j.jnutbio.2011.05.015

Chen, T., Chen, H., Wang, Y., & Zhang, J. (2016). In vitro and in vivo antitumour activities of puerarin 6''-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway. Pharm Biol, 54(9), 1793-1799. doi:10.3109/13880209.2015.1127980

Cherdshewasart, W., Subtang, S., & Dahlan, W. (2007). Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata. J Pharm Biomed Anal, 43(2), 428-434. doi:10.1016/j.jpba.2006.07.013

Commission, C. P. (2010). A Colored Identification Atlas of Chinese Materia Medica and Plants as Specified in the Pharmacopoeia of the People's Republic of China: People's Medical Publishing House.

Gan, M., & Yin, X. (2015). Puerarin induced in mantle cell lymphoma apoptosis and its possible mechanisms involving multi-signaling pathway. Cell Biochem Biophys, 71(1), 367-373. doi:10.1007/s12013-014-0207-y

Han, J., Yu, C. Q., & Shen, W. (2009). [Inhibitory effects of puerarin on invasion and metastasis of oophoroma cells HO-8910]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 29(7), 632-635.

Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacol Ther, 96(2-3), 67-202.

Hien, T. T., Kim, H. G., Han, E. H., Kang, K. W., & Jeong, H. G. (2010). Molecular mechanism of suppression of MDR1 by puerarin from Pueraria lobata via NF-kappaB pathway and cAMP-responsive element transcriptional activity-dependent up-regulation of AMP-activated protein kinase in breast cancer MCF-7/adr cells. Mol Nutr Food Res, 54(7), 918-928. doi:10.1002/mnfr.200900146

Hu, Y., Li, X., Lin, L., Liang, S., & Yan, J. (2018). Puerarin inhibits non-small cell lung cancer cell growth via the induction of apoptosis. Oncol Rep. doi:10.3892/or.2018.6234

Hu, Y., Liu, K., Bo, S., Yan, M., Zhang, Y., Miao, C., & Ren, L. (2016). Inhibitory effect of puerarin on vascular smooth muscle cells proliferation induced by oxidised low-density lipoprotein via suppressing ERK 1/2 phosphorylation and PCNA expression. Pharmazie, 71(2), 89-93.

Hughes, C. L., Jr. (1988). Phytochemical mimicry of reproductive hormones and modulation of herbivore fertility by phytoestrogens. Environ Health Perspect, 78, 171-174.

Jiang, K., Chen, H., Tang, K., Guan, W., Zhou, H., Guo, X., . . . Xu, H. (2018). Puerarin inhibits bladder cancer cell proliferation through the mTOR/p70S6K signaling pathway. Oncol Lett, 15(1), 167-174. doi:10.3892/ol.2017.7298

Jun, M., Hong, J., Jeong, W. S., & Ho, C. T. (2005). Suppression of arachidonic acid metabolism and nitric oxide formation by kudzu isoflavones in murine macrophages. Mol Nutr Food Res, 49(12), 1154-1159. doi:10.1002/mnfr.200500103

Kang, H., Zhang, J., Wang, B., Liu, M., Zhao, J., Yang, M., & Li, Y. (2017). Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathway. Int J Oncol, 50(2), 545-554. doi:10.3892/ijo.2017.3841

Kanwal, R., & Gupta, S. (2012). Epigenetic modifications in cancer. Clin Genet, 81(4), 303-311. doi:10.1111/j.1399-0004.2011.01809.x

Kitano, H. (2002). Systems biology: a brief overview. Science, 295(5560), 1662-1664. doi:10.1126/science.1069492

Klejdus, B., Mikelova, R., Petrlova, J., Potesil, D., Adam, V., Stiborova, M., . . . Kuban, V. (2005). Evaluation of isoflavone aglycon and glycoside distribution in soy plants and soybeans by fast column high-performance liquid chromatography coupled with a diode-array detector. J Agric Food Chem, 53(15), 5848-5852. doi:10.1021/jf0502754

Ko, K. P. (2014). Isoflavones: chemistry, analysis, functions and effects on health and cancer. Asian Pac J Cancer Prev, 15(17), 7001-7010.

Korkina, L. G. (2007). Phenylpropanoids as naturally occurring antioxidants: from plant defense to human health. Cell Mol Biol (Noisy-le-grand), 53(1), 15-25.

le Sage, C., & Agami, R. (2006). Immense promises for tiny molecules: uncovering miRNA functions. Cell Cycle, 5(13), 1415-1421. doi:10.4161/cc.5.13.2890

Li, J., Lin, H., Hou, W., & Hua, B. (2010). Idea and strategy of traditional Chinese medicine treatment for cancer. China Cancer, 19(11), 735-738.

Lin, F., Xie, B., Cai, F., & Wu, G. (2012). Protective effect of Puerarin on beta-amyloid-induced neurotoxicity in rat hippocampal neurons. Arzneimittelforschung, 62(4), 187-193. doi:10.1055/s-0031-1299763

Lin, Y. J., Hou, Y. C., Lin, C. H., Hsu, Y. A., Sheu, J. J., Lai, C. H., . . . Tsai, F. J. (2009). Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells. Biochem Biophys Res Commun, 378(4), 683-688. doi:10.1016/j.bbrc.2008.10.178

Liu, X., Li, S., Li, Y., Cheng, B., Tan, B., & Wang, G. (2018). Puerarin inhibits proliferation and induces apoptosis by up-regulation of miR-16 in bladder cancer cell line T24. Oncol Res. doi:10.3727/096504018x15178736525106

Liu, X., Zhao, W., Wang, W., Lin, S., & Yang, L. (2017). Puerarin suppresses LPS-induced breast cancer cell migration, invasion and adhesion by blockage NF-kappaB and Erk pathway. Biomed Pharmacother, 92, 429-436. doi:10.1016/j.biopha.2017.05.102

Luo, J., Qu, J., Yang, R., Ge, M. X., Mei, Y., Zhou, B. T., & Qu, Q. (2016). Phytochemicals Mediate the Expression and Activity of OCTN2 as Activators of the PPARgamma/RXRalpha Pathway. Front Pharmacol, 7, 189. doi:10.3389/fphar.2016.00189

Maji, A. K., Pandit, S., Banerji, P., & Banerjee, D. (2014). Pueraria tuberosa: a review on its phytochemical and therapeutic potential. Nat Prod Res, 28(23), 2111-2127. doi:10.1080/14786419.2014.928291

Moon, Y. J., Wang, X., & Morris, M. E. (2006). Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. Toxicol In Vitro, 20(2), 187-210. doi:10.1016/j.tiv.2005.06.048

Pan, Z. Y., Bao, Z. S., Wu, Z. M., Wang, X. M., Zheng, J. Z., Shen, Y. L., & Zhang, X. M. (2009). The myocardial protective effects of puerarin on STZ-induced diabetic rats. Fen Zi Xi Bao Sheng Wu Xue Bao, 42(2), 137-144.

Penetar, D. M., Toto, L. H., Farmer, S. L., Lee, D. Y., Ma, Z., Liu, Y., & Lukas, S. E. (2012). The isoflavone puerarin reduces alcohol intake in heavy drinkers: a pilot study. Drug Alcohol Depend, 126(1-2), 251-256. doi:10.1016/j.drugalcdep.2012.04.012

Peng, X. Y., Qi, Z. H., & Chen, H. P. (2001). [Study on the differentiation and apoptosis of HL-60 cell line induced by Puerarin]. Hunan Yi Ke Da Xue Xue Bao, 26(2), 126-128.

Pengyu S , A. B., Lijuan Z. (2018). Natural β-Elemene:Advances in Targeting Cancer Through DifferentMolecular Pathways. North American Journal of Acedamic Research, 1(4), 27.

Prasad, S., Phromnoi, K., Yadav, V. R., Chaturvedi, M. M., & Aggarwal, B. B. (2010). Targeting inflammatory pathways by flavonoids for prevention and treatment of cancer. Planta Med, 76(11), 1044-1063. doi:10.1055/s-0030-1250111

Prasain, J. K., Jones, K., Kirk, M., Wilson, L., Smith-Johnson, M., Weaver, C., & Barnes, S. (2003). Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry. J Agric Food Chem, 51(15), 4213-4218. doi:10.1021/jf030174a

Shao, H. M., Tang, Y. H., Jiang, P. J., Zhu, H. Q., Zhang, Y. C., Ji, J. M., . . . Shen, Q. (2010). [Inhibitory effect of flavonoids of puerarin on proliferation of different human acute myeloid leukemia cell lines in vitro]. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 18(2), 296-299.

Sharma, S. K., Kumar, S., Chand, K., Kathuria, A., Gupta, A., & Jain, R. (2011). An update on natural occurrence and biological activity of chromones. Curr Med Chem, 18(25), 3825-3852.

Sun, F., & Sun, X. (1999). Sophora flavescens. Shen Nong Ben Cao Jing. Scientific and Technical Documents Publishing House, p59.

Tan, Y., Liu, M., & Wu, B. (2008). Puerarin for acute ischaemic stroke. Cochrane Database Syst Rev(1), Cd004955. doi:10.1002/14651858.CD004955.pub2

Tang, J., Guo, F., Du, Y., Liu, X., Qin, Q., Liu, X., . . . Wang, Y. (2015). Continuous exposure of non-small cell lung cancer cells with wild-type EGFR to an inhibitor of EGFR tyrosine kinase induces chemoresistance by activating STAT3. Int J Oncol, 46(5), 2083-2095. doi:10.3892/ijo.2015.2898

Tang, Y. H., Zhu, H. Q., Zhang, Y. C., Shao, H. M., Ji, J. M., Zhu, G. R., . . . Shen, Q. (2010). [Apoptosis of NB4 cells induced by flavonoids of puerarin in vitro]. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 18(2), 326-329.

Wang, J., Yang, Z. R., Guo, X. F., Song, J., Zhang, J. X., Wang, J., & Dong, W. G. (2014). Synergistic effects of puerarin combined with 5-fluorouracil on esophageal cancer. Mol Med Rep, 10(5), 2535-2541. doi:10.3892/mmr.2014.2539

Wang, Q., Wu, T., Chen, X., Ni, J., Duan, X., Zheng, J., . . . Wei, J. (2006). Puerarin injection for unstable angina pectoris. Cochrane Database Syst Rev(3), Cd004196. doi:10.1002/14651858.CD004196.pub2

Wang, Y., Ma, Y., Zheng, Y., Song, J., Yang, X., Bi, C., . . . Zhang, Q. (2013). In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity. Int J Pharm, 441(1-2), 728-735. doi:10.1016/j.ijpharm.2012.10.021

Wei, S. Y., Chen, Y., & Xu, X. Y. (2014). Progress on the pharmacological research of puerarin: a review. Chin J Nat Med, 12(6), 407-414. doi:10.1016/s1875-5364(14)60064-9

Wong, K. H., Li, G. Q., Li, K. M., Razmovski-Naumovski, V., & Chan, K. (2011). Kudzu root: traditional uses and potential medicinal benefits in diabetes and cardiovascular diseases. J Ethnopharmacol, 134(3), 584-607. doi:10.1016/j.jep.2011.02.001

Wong, R., & Rabie, B. (2007). Effect of puerarin on bone formation. Osteoarthritis Cartilage, 15(8), 894-899. doi:10.1016/j.joca.2007.02.009

Yao, X. J., Yin, J. A., Xia, Y. F., Wei, Z. F., Luo, Y. B., Liu, M., . . . Dai, Y. (2012). Puerarin exerts antipyretic effect on lipopolysaccharide-induced fever in rats involving inhibition of pyrogen production from macrophages. J Ethnopharmacol, 141(1), 322-330. doi:10.1016/j.jep.2012.02.038

Yin, S. Y., Wei, W. C., Jian, F. Y., & Yang, N. S. (2013). Therapeutic applications of herbal medicines for cancer patients. Evid Based Complement Alternat Med, 2013, 302426. doi:10.1155/2013/302426

Yu, C., Li, Y., Chen, H., Yang, S., & Xie, G. (2008). Decreased expression of aromatase in the Ishikawa and RL95-2 cells by the isoflavone, puerarin, is associated with inhibition of c-jun expression and AP-1 activity. Food Chem Toxicol, 46(12), 3671-3676. doi:10.1016/j.fct.2008.09.045

Yu, Z., & Li, W. (2006). Induction of apoptosis by puerarin in colon cancer HT-29 cells. Cancer Lett, 238(1), 53-60. doi:10.1016/j.canlet.2005.06.022

Zeng, Y. P., Yang, Z. R., Guo, X. F., Jun, W., & Dong, W. G. (2014). Synergistic effect of puerarin and 5-fluorouracil on hepatocellular carcinoma. Oncol Lett, 8(6), 2436-2442. doi:10.3892/ol.2014.2534

Zhang, H., Zhai, Z., Zhou, H., Li, Y., Li, X., Lin, Y., . . . Zhou, M. S. (2015). Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage. Biomed Res Int, 2015, 403616. doi:10.1155/2015/403616

Zhang, W. G., Liu, X. F., Meng, K. W., & Hu, S. Y. (2014). Puerarin inhibits growth and induces apoptosis in SMMC-7721 hepatocellular carcinoma cells. Mol Med Rep, 10(5), 2752-2758. doi:10.3892/mmr.2014.2512

Zhang, W. G., Yin, X. C., Liu, X. F., Meng, K. W., Tang, K., Huang, F. L., . . . Gao, J. (2017). Puerarin Induces Hepatocellular Carcinoma Cell Apoptosis Modulated by MAPK Signaling Pathways in a Dose-dependent Manner. Anticancer Res, 37(8), 4425-4431. doi:10.21873/anticanres.11837

Zhang, Z., Lam, T. N., & Zuo, Z. (2013). Radix Puerariae: an overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J Clin Pharmacol, 53(8), 787-811. doi:10.1002/jcph.96

Zhao, M., Du, Y. Q., Yuan, L., & Wang, N. N. (2010). Protective effect of puerarin on acute alcoholic liver injury. Am J Chin Med, 38(2), 241-249. doi:10.1142/s0192415x10007816


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